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Solution structure of the active-centre mutant I14A of the histidine-containing phosphocarrier protein from Staphylococcus carnosus

Möglich, Andreas ; Koch, Brigitte ; Gronwald, Wolfram ; Hengstenberg, Wolfgang ; Brunner, Eike ; Kalbitzer, Hans Robert



Zusammenfassung

High-pressure NMR experiments performed on the histidine-containing phosphocarrier protein (HPr) from Staphylococcus carnosus have shown that residue Ile14, which is located in the active-centre loop, exhibits a peculiarly small pressure response. In contrast, the rest of the loop shows strong pressure effects as is expected for typical protein interaction sites. To elucidate the structural role ...

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