Flüssigkeits-Chromatographie an Triacetylcellulose 4. Determination of enantiomeric purity in spite of incomplete chromatographic separation

Abstract

A novel procedure for the measurement of enantiomeric purity P is derived, whereby the mixture to be analyzed is subjected to liquid chromatography (LC) on an optically active sorbent. Extensive peak overlap does not prohibit the application of the method, whereas the complete absence of separation does. In addition, the mixture is subjected to LC on an achiral sorbent. The same photometer and polarimeter detections are used for both experiments. At the same time, an x/y recorder plots the rotation angle α versus the absorbance A, thus generating the slopes Cm and C+ in Fig. 1 which result in P = Cm/C+. – Optically active samples of 1, 2, 3, and 4 were analyzed for P. The findings agree satisfactorily with the results of other methods.