Song, S. and Dick, Bernhard and Penzkofer, Alfons (2007) Photo-induced reduction of flavin mononucleotide in aqueous solutions. Chemical Physics 332 (1), pp. 55-65.
Download (551kB) - Repository staff only
Other URL: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TFM-4MF3Y21-2&_user=616165&_coverDate=01%2F25%2F2007&_alid=620794472&_rdoc=3&_fmt=summary&_orig=search&_cdi=5230&_sort=d&_docanchor=&view=c&_ct=9&_acct=C000032338&_version=1&_urlVersion=0&_userid=
The photo-induced reduction of flavin mononucleotide (FMN) in aqueous solutions is studied by absorption spectra measurement under aerobic and anaerobic conditions. Samples without exogenous reducing agent and with the exogenous reducing agents ethylene-diamine-tetraacetic acid (EDTA) and dithiothreitol (DTT) are investigated. Under anaerobic conditions the photo-induced reduction with and without reducing agents is irreversible. Under aerobic conditions the photo-reduction without added reducing agent is small compared to the photo-degradation, and the photo-reduction of FMN by the reducing agents is reversible (re-oxidation in the dark). During photo-excitation of FMN the dissolved oxygen is consumed by singlet oxygen formation and subsequent chemical reaction. After light switch-off slow re-oxidation (slow absorption recovery) occurs due to air in-diffusion from surface. EDTA degradation by FMN excitation leads to oxygen scavenging. The quantum efficiencies of photo-reduction under aerobic and anaerobic conditions are determined. The re-oxidation of reduced FMN under aerobic conditions and due to air injection is investigated.
|Institutions:|| Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry III - Physical Chemistry (Light and Matter) > Prof. Dr. Bernhard Dick |
Physics > Institute of Experimental and Applied Physics > Retired Professors > Group Alfons Penzkofer
|Projects:||GRK 640 Sensory photoreceptors in natural and artificial systems, Blaulicht-sensitive Photorezeptoren|
|Keywords:||Flavin mononucleotide (FMN); Ethylene-diamine-tetraacetic acid (EDTA); Dithiothreitol (DTT); Flavin photo-reduction; Aerobic photo-reduction; Anaerobic photo-reduction; Absorption spectroscopy; Flavin re-oxidation; Oxygen scavenging|
|Subjects:||500 Science > 530 Physics|
500 Science > 540 Chemistry & allied sciences
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Yes|
|Owner:||Prof. Dr. Bernhard Dick|
|Deposited On:||20 Mar 2007|
|Last Modified:||27 Aug 2012 14:13|