Liebl, B. and Pachmann, K. and Römer, U. and Haen, Ekkehard and Hallek, M. and Emmerich, B.
Quantitation of mRNA for the beta-adrenoceptor gene in human mononuclear leucocytes by in situ hybridization with fluorochrome labeled cloned DNA.
Journal of receptor research 11 (1-4), pp. 473-482.
Determination of the transcriptional activity of the beta-adrenoceptor (beta-AR) gene in human mononuclear leucocytes (MNL) may provide important information concerning variations in receptor expression in clinical studies. We used a new method with highly fluorochrome-labeled gene probes for in situ hybridization which allows a rapid detection of mRNA at the cellular level. MNL from venous blood of healthy volunteers were permeabilized, fixed and attached to adhesive glass slides. After incubation with fluorochrome-labeled cloned hamster beta AR-DNA probes the amount of DNA-mRNA hybrids formed in the cytoplasm of individual cells was analyzed by microfluorimetry of specifically bound fluorochrome. In nine of ten normal subjects studied so far a fraction of beta AR-mRNA positive MNL could be detected. The proportion of positive cells as well as their signal intensity revealed a high interindividual variance.
|Institutions:||Medicine > Lehrstuhl für Psychiatrie und Psychotherapie|
|Nucleic Acid Hybridization||MESH|
|Receptors, Adrenergic, beta/genetics||MESH|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||18 Feb 2011 10:23|
|Last Modified:||13 Mar 2014 16:54|