Achatz, E. and Heiligtag, Florian J. and Li, Xiaohua and Link, Martin and Wolfbeis, Otto S. (2010) Colloidal silica nanoparticles for use in click chemistry-based conjugations and fluorescent affinity assays. Sensors and Actuators B: Chemical 150 (1), pp. 211-219.
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Silica nanoparticles (SiNPs) with an average diameter of 25 nm were prepared by a one-pot method that involves the formation of a silica core that is subsequently covered with a shell functionalized with either azido groups or alkyne groups for use in copper(I)-catalyzed click reactions. Respective triethoxysilane reagents are presented. The SiNPs were (a) rendered fluorescent by click conjugation to fluorophores of various colors, and (b) made bioconjugatable by introducing maleimide groups (that covalently bind thiols) and biotin (a widely used bioaffinity reagent that binds streptavidin). Particles were characterized by transmission electron microscopy, infrared spectroscopy, fluorescence, and light scattering. The fluorescently labeled SiNPs carrying maleimido groups were conjugated to the thiol group of bovine serum albumin (BSA) labeled with a fluorophore, and fluorescence resonance energy transfer was shown to occur between the labeled SiNPs and the labeled BSA. This is considered to represent a new approach towards nanoparticle-based fluorescent bioassays.
|Date:||21 September 2010|
|Institutions:||Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Otto S. Wolfbeis)|
|Keywords:||Silica nanoparticles; Surface chemistry; Fluorescence; Bioconjugation; Affinity sensing; Biotin; Click chemistry|
|Subjects:||500 Science > 540 Chemistry & allied sciences|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||18 Mar 2011 06:47|
|Last Modified:||21 Jul 2011 02:08|