Kocincová, Anna S. and Nagl, Stefan and Arain, Sarina and Krause, Christian and Borisov, Sergey M. and Arnold, Matthias and Wolfbeis, Otto S. (2008) Multiplex bacterial growth monitoring in 24-well microplates using a dual optical sensor for dissolved oxygen and pH. Biotechnology and Bioengineering 100 (3), pp. 430-438.
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Non-invasive, simultaneous optical monitoring of oxygen and pH during bacterial cultivation in 24-well microplates is presented using an integrated dual sensor for dissolved oxygen and pH values. The dual sensor is based on oxygen-sensitive organosilica microparticles and pH-sensitive microbeads from a polymethacrylate derivative embedded into a polyurethane hydrogel. The readout is based on a phase-domain fluorescence lifetime-based method referred to as modified frequency domain dual lifetime referencing using a commercially available detector system for 24-well microplates. The sensor was used for monitoring the growth of Pseudomonas putida bacterial cultures. The method is suitable for parallelized, miniaturized bioprocessing, and cell-based high-throughput screening applications. Biotechnol. Bioeng. 2008;100: 430–438. © 2008 Wiley Periodicals, Inc.
|Date:||15 June 2008|
|Institutions:||Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Otto S. Wolfbeis)|
|Keywords:||oxygen sensing; pH sensing; microbioreactor; bioprocess; dual sensor; fluorescence|
|Subjects:||500 Science > 540 Chemistry & allied sciences|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||18 Mar 2011 07:30|
|Last Modified:||21 Jul 2011 02:09|