Arain, Sarina and John, Gernot T. and Krause, Christian and Gerlach, Jochen and Wolfbeis, Otto S. and Klimant, Ingo (2006) Characterization of microtiterplates with integrated optical sensors for oxygen and pH, and their applications to enzyme activity screening, respirometry, and toxicological assays. Sensors and Actuators B Chemical 113 (2), pp. 639-648.
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This paper describes properties of microplates (MTPs) with integrated, fluorescence-based sensors for pH and oxygen, and their application for enzyme screening and monitoring of bacterial respiratory activity. Thin, hydrophilic sensing films consisting of an analyte-sensitive indicator and a reference fluorophore, are deposited on the bottom of the MTP. This allows for calibration-free quantification of pH and pO2 with an acceptable accuracy and resolution for applications such as enzyme activity screening, respirometry, or toxicological assays. The sensor properties are mainly investigated with respect to their qualification for such applications. Specifically, enzyme activity screening is demonstrated for glucose oxidase using oxygen-sensitive plates, and for bacterial growth monitoring of Escherichia coli and Pseudomonas putida. Furthermore, a toxicological assay monitoring the respiration activity of P. putida was converted into a microplate format.
|Date:||27 February 2006|
|Institutions:||Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Otto S. Wolfbeis)|
|Keywords:||Optical oxygen sensor; Optical pH sensor; MTP; Enzyme activity; Respiratory activity|
|Subjects:||500 Science > 540 Chemistry & allied sciences|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||04 Apr 2011 10:29|
|Last Modified:||21 Jul 2011 02:10|