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Characterization of microtiterplates with integrated optical sensors for oxygen and pH, and their applications to enzyme activity screening, respirometry, and toxicological assays

Arain, Sarina and John, Gernot T. and Krause, Christian and Gerlach, Jochen and Wolfbeis, Otto S. and Klimant, Ingo (2006) Characterization of microtiterplates with integrated optical sensors for oxygen and pH, and their applications to enzyme activity screening, respirometry, and toxicological assays. Sensors and Actuators B Chemical 113 (2), pp. 639-648.

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Abstract

This paper describes properties of microplates (MTPs) with integrated, fluorescence-based sensors for pH and oxygen, and their application for enzyme screening and monitoring of bacterial respiratory activity. Thin, hydrophilic sensing films consisting of an analyte-sensitive indicator and a reference fluorophore, are deposited on the bottom of the MTP. This allows for calibration-free ...

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Item Type:Article
Date:27 February 2006
Institutions:Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Otto S. Wolfbeis)
Identification Number:
ValueType
10.1016/j.snb.2005.07.056DOI
Keywords:Optical oxygen sensor; Optical pH sensor; MTP; Enzyme activity; Respiratory activity
Subjects:500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Owner: Martin Kaiser
Deposited On:04 Apr 2011 10:29
Last Modified:13 Mar 2014 17:07
Item ID:20294
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