Babilas, Philipp and Liebsch, Gregor and Schacht, Vivien and Klimant, Ingo and Wolfbeis, Otto S. and Szeimies, Rolf-Markus and Abels, Christoph (2005) In Vivo Phosphorescence Imaging of pO2 Using Planar Oxygen Sensors. Microcirculation 12 (6), pp. 477-487.
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Objective: Oxygen-dependent quenching of luminescence of metal porphyrin complexes has been used to image the pO2 distribution over tumor and normal tissue.
Methods: An experimental setup is described using a platinum(II)–octaethyl–porphyrin immobilized in a polystyrene matrix as transparent planar sensor.
Results: Sensitivity over a broad range is high at low pO2 values (± 0.2 mm Hg at 0 mm Hg; ± 1.5 mm Hg at 160 mm Hg pO2). Due to intrinsically referencing via lifetime encoding there was no modification of the sensor response in vivo in the dorsal skinfold chamber model with amelanotic melanoma (A-MEL-3) in awake hamsters when compared to the in vitro calibration. pO2 measurements over normal tissue (25.8 ± 5.1 mm Hg) and tumor tissue (9.2 ± 5.1 mm Hg) were in excellent agreement with previous results obtained in this model using a surface multiwire electrode.
Conclusions: Using the presented method the surface pO2 distribution can be mapped with a high temporal resolution of approximately 100 ms and a spatial resolution of at least 25 μ m. Moreover, the transparent sensor allows the simultaneous visualization of the underlying microvasculature.
|Institutions:||Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Otto S. Wolfbeis)|
|Keywords:||dorsal skinfold chamber; intravital microscopy; microcirculation; oxygen; temperature|
|Subjects:||500 Science > 540 Chemistry & allied sciences|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||04 Apr 2011 10:37|
|Last Modified:||13 Mar 2014 17:09|