Gruber, Michaela and Wetzl, Bianca and Oswald, Bernhard and Enderlein, Jörg and Wolfbeis, Otto S. (2005) A New Fluorescence Resonance Energy Transfer Pair and Its Application to Oligonucleotide Labeling and Fluorescence Resonance Energy Transfer Hybridization Studies. Journal of Fluorescence 15 (3), pp. 207-214.
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We describe two new fluorescence resonance energy transfer (FRET) compatible labels, their covalent linkage to oligonucleotides, and their use as donor and acceptor, respectively, in FRET hybridization studies. The dyes belong to the cyanine dyes, and water solubility is imparted by a phosphonate which represents a new solubilizing group in DNA labels. They were linked to amino-modified synthetic oligonucleotides via oxysuccinimide (OSI) esters. The studies performed include binding assays, determinations of molecular distances, homogeneous competitive assays, and limits of detection, which are in the order of 5 pmol/L for a 15-mer.
|Institutions:||Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Otto S. Wolfbeis)|
|Keywords:||Fluorescence hybridization assay - fluorescence resonance energy transfer - long-wavelength fluorescent labels|
|Subjects:||500 Science > 540 Chemistry & allied sciences|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||04 Apr 2011 10:48|
|Last Modified:||21 Jul 2011 02:10|