Langford, A. and Kunze, R. and Schmelzer, S. and Wolf, Hans J. and Pohle, H. D. and Reichart, P.
Immunocytochemical detection of herpes viruses in oral smears of HIV-infected patients.
Journal of oral pathology & medicine 21 (2), pp. 49-57.
Cytologic smears (CS) were taken from the lateral border of the tongue of HIV-seropositive patients (HIV+) (n = 39) and of seronegative controls (HIV-) (n = 19) and examined by immunocytochemistry (APAAP) and in situ hybridization (ISH) (biotinylated DNA probes) for the presence of viral antigens/DNA of EBV and CMV. While none of the HIV controls showed positive results for EBV antigen, 61% (APAAP) resp. 79% (ISH) of oral epithelial cells in the group of HIV+ patients were EBV-positive. While all CS taken from areas with the clinical diagnosis of hairy leukoplakia (HL) were EBV positive (APAAP and/or ISH), the detection of EBV in CS from uninvolved oral mucosa seemed to be associated with the later development of HL. In the group of HIV+ patients the detection rate for CMV was about five times (APAAP) resp. three times (ISH) higher than in HIV- persons. This non-invasive technique seems to be a valuable tool to screen for viral antigens/genomes.
|Institutions:|| Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene|
|Cytomegalovirus/isolation & purification||MESH|
|Herpesvirus 4, Human/isolation & purification||MESH|
|Nucleic Acid Hybridization||MESH|
|Tumor Virus Infections/microbiology||MESH|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||12 Apr 2011 03:18|
|Last Modified:||21 Jul 2011 02:11|