Marschall, M. and Schwarzmann, F. and Leser, U. and Oker, B. and Alliger, P. and Mairhofer, H. and Wolf, Hans J.
The BI'LF4 trans-activator of Epstein-Barr virus is modulated by type and differentiation of the host cell.
Virology 181 (1), pp. 172-179.
We have analyzed the activity and regulated expression of a new Epstein-Barr virus (EBV) trans-activator (I'ta) encoded by left reading frame 4 (BI'LF4) of the BamHI I'fragment. The gene was detected in all genomes of established EBV strains and individual isolates, with the exception of B95-8, where the type-specific deletion of this open reading frame is tolerated in vitro. Specific trans-activation of two EBV promoters (early MS and I'ta promoter) could be shown in cotransfection assays. The I'ta product affected autoactivation but had no influence on heterologous target promoters. The I'ta promoter segment was shown to be costimulated in the process of host cell differentiation in the absence of other EBV gene products. Expression of the reading frame in bacteria identified a 48-kDa protein as a stable gene product. I'ta-specific antibodies were detected in sera from EBV-positive persons (nasopharyngeal carcinoma). When expressed with suitable eucaryotic vectors, a nuclear protein could be immunostained in transfected cells. Our experiments suggest a cell type-specific requirement for I'ta in the lytic cycle of EBV at a determined differentiation stage of the host cell.
|Institutions:|| Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene|
|Fluorescent Antibody Technique||MESH|
|Gene Expression Regulation, Viral||MESH|
|Hepatitis B Surface Antigens/genetics||MESH|
|Herpesvirus 4, Human/genetics||MESH|
|Recombinant Fusion Proteins/metabolism||MESH|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||12 Apr 2011 07:54|
|Last Modified:||21 Jul 2011 02:11|