Schwarz, T. F. and Modrow, Susanne and Hottenträger, B. and Höflacher, B. and Jäger, G. and Scharti, W. and Sumazakl, R. and Wolf, Hans J. and Middeldorp, J. and Roggendorf, M.
New oligopeptide immunoglobulin G test for human parvovirus B19 antibodies.
Journal of clinical microbiology 29 (3), pp. 431-435.
A new, highly sensitive and specific enzyme immunoassay using oligopeptides as antigen (enzyme-linked immunosorbent assay [ELISA] B19-OP) for detecting parvovirus B19-specific immunoglobulin G (IgG) was established. As antigens, B19-specific oligopeptides of 24 and 30 kDa derived from a 196-kDa fusion protein of beta-galactosidase and viral capsid protein (VPI) of B19 after CNBr cleavage and separation by high-pressure liquid chromatography were used. Of 139 serum specimens tested in parallel for anti-B19 IgG by standard ELISA using B19 particles as antigen and by ELISA B19-OP, 73 (52.5%) were positive and 63 (45.3%) were negative in both tests, and 3 (2.2%) were negative by standard ELISA but positive by ELISA B19-OP and by immunoblot. By using ELISA B19-OP, it was possible to detect anti-B19 IgG in an asymptomatic blood donor 4 weeks after acute infection, and anti-B19 IgG titers of 10(-5) could be measured in convalescent-phase sera.
|Institutions:|| Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene|
|Enzyme-Linked Immunosorbent Assay||MESH|
|Sensitivity and Specificity||MESH|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||12 Apr 2011 08:02|
|Last Modified:||21 Jul 2011 02:11|