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Detection of Epstein-Barr virus DNA in nasopharyngeal carcinoma using a non-radioactive digoxigenin-labelled probe

Permeen, A. M. and Sam, C. K. and Pathmanathan, R. and Prasad, U. and Wolf, Hans J. (1990) Detection of Epstein-Barr virus DNA in nasopharyngeal carcinoma using a non-radioactive digoxigenin-labelled probe. Journal of virological methods 27 (3), pp. 261-267.

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Abstract

The presence of Epstein Barr virus (EBV) DNA in biopsies from the post-nasal space (PNS) of patients suspected of nasopharyngeal carcinoma (NPC) was detected by in situ cytohybridization with an EBV DNA probe labelled with the novel labelling compound digoxigenin. The digoxigenin probe was hybridised to cryostat sections of NPC biopsies and subsequently detected by an enzyme immunoassay ...

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Item Type:Article
Date:1990
Institutions:Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene
Identification Number:
ValueType
2157729PubMed ID
10.1016/0166-0934(90)90094-VDOI
Classification:
NotationType
Cell LineMESH
DNA ProbesMESH
DNA, Viral/analysisMESH
Digoxigenin/diagnostic useMESH
Digoxin/analogs & derivativesMESH
False Positive ReactionsMESH
Herpesvirus 4, Human/geneticsMESH
Immunoenzyme TechniquesMESH
Nasopharyngeal Neoplasms/microbiologyMESH
Nucleic Acid HybridizationMESH
Specimen HandlingMESH
Tritium/diagnostic useMESH
Subjects:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Owner: Universitätsbibliothek Regensburg
Deposited On:13 Apr 2011 08:28
Last Modified:13 Mar 2014 17:15
Item ID:20487
Owner Only: item control page

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