Sumazaki, R. and Motz, M. and Wolf, Hans and Heinig, J. and Jilg, Wolfgang and Deinhardt, F.
Detection of hepatitis B virus in serum using amplification of viral DNA by means of the polymerase chain reaction.
Journal of medical virology 27 (4), pp. 304-308.
A new assay was developed for the detection of hepatitis B virus (HBV) in human serum using amplification of a short viral DNA sequence by means of the polymerase chain reaction. As little as 0.4 fg viral DNA, corresponding to about 130 genome equivalents, per ml serum could be detected after the amplification procedure. This assay detected viral DNA in a number of patients with proven or suspected chronic HBV infection who were all negative for HBV DNA in the conventional hybridisation assay. We found HBV DNA in all of six HBeAg-positive and in three of eight HBeAg-negative HBsAg carriers, as well as in all of 11 patients with chronic liver disease with antibodies against the HBV core antigen (anti-HBc) as the sole marker for HBV infection, and in three of five apparently healthy individuals showing only anti HBc. Thus, this method is an important improvement for the diagnosis of persistent HBV infections, especially in patients where a definitive serological diagnosis is not possible.
|Institutions:|| Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene|
|DNA-Directed DNA Polymerase/metabolism||MESH|
|Electrophoresis, Agar Gel||MESH|
|Hepatitis B Antigens/immunology||MESH|
|Hepatitis B virus/isolation & purification||MESH|
|Sensitivity and Specificity||MESH|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||13 Apr 2011 08:45|
|Last Modified:||21 Jul 2011 02:11|