Startseite UB

Detection of hepatitis B virus in serum using amplification of viral DNA by means of the polymerase chain reaction

Sumazaki, R. and Motz, M. and Wolf, Hans and Heinig, J. and Jilg, Wolfgang and Deinhardt, F. (1989) Detection of hepatitis B virus in serum using amplification of viral DNA by means of the polymerase chain reaction. Journal of medical virology 27 (4), pp. 304-308.

[img]
Preview
PDF
Download (453kB)

at PubMed


Abstract

A new assay was developed for the detection of hepatitis B virus (HBV) in human serum using amplification of a short viral DNA sequence by means of the polymerase chain reaction. As little as 0.4 fg viral DNA, corresponding to about 130 genome equivalents, per ml serum could be detected after the amplification procedure. This assay detected viral DNA in a number of patients with proven or ...

plus


Export bibliographical data



Item Type:Article
Date:1989
Institutions:Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene
Identification Number:
ValueType
2656910PubMed ID
Classification:
NotationType
Antibodies, Viral/immunologyMESH
Blotting, SouthernMESH
DNA ProbesMESH
DNA, Viral/biosynthesisMESH
DNA-Directed DNA Polymerase/metabolismMESH
Electrophoresis, Agar GelMESH
Gene AmplificationMESH
Hepatitis Antibodies/analysisMESH
Hepatitis B/diagnosisMESH
Hepatitis B Antigens/immunologyMESH
Hepatitis B virus/isolation & purificationMESH
HumansMESH
Immune Sera/analysisMESH
MethodsMESH
PlasmidsMESH
Sensitivity and SpecificityMESH
Serologic TestsMESH
Taq PolymeraseMESH
Subjects:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Owner: Universitätsbibliothek Regensburg
Deposited On:13 Apr 2011 08:45
Last Modified:13 Mar 2014 17:15
Item ID:20490
Owner Only: item control page

Downloads

Downloads per month over past year

  1. University

University Library

Publication Server

Contact person
Gernot Deinzer

Telefon 0941 943-2759
Contact