Modrow, Susanne and Höflacher, B. and Mertz, R. and Wolf, Hans J.
Carrier-bound synthetic peptides. Use as antigen in HIV-1 ELISA tests and in antiserum production.
Journal of immunological methods 118 (1), pp. 1-7.
Chemically synthesize carrier-bound peptides have been used as antigens in diagnostic test systems (ELISA) and for raising antipeptide-specific antisera. The method does not require prior cleavage of the peptides from the support used for the solid-phase synthesis. Using the same resin for both the synthesis and the subsequent applications it was possible to avoid expensive and time-consuming purification procedures and artificial recoupling to solid supports. A quick and specific ELISA-based diagnostic test system for HIV-specific antipeptide antibodies in human sera was established. In addition the carrier-bound peptides were shown to be potent antigens for raising antibodies in animals.
|Institutions:|| Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene|
|Enzyme-Linked Immunosorbent Assay/methods||MESH|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||15 Apr 2011 09:14|
|Last Modified:||21 Jul 2011 02:11|