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Truncated versions of the two major Epstein-Barr viral glycoproteins (gp250/350) are secreted by recombinant Chinese hamster ovary cells

Motz, M. and Deby, G. and Wolf, Hans J. (1987) Truncated versions of the two major Epstein-Barr viral glycoproteins (gp250/350) are secreted by recombinant Chinese hamster ovary cells. Gene 58 (1), pp. 149-154.

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Abstract

The expression of the two Epstein-Barr virus (EBV) major membrane proteins gp250/350 (MA-BLLF1) on the surface of recombinant CHO clones cannot be amplified by methotrexate (MTX) selection, perhaps due to toxic effects of these membrane proteins. After removal of sequences encoding the part of the glycoproteins responsible for membrane anchorage, the gp250/350 is secreted into the medium. Following selection with MTX, this construct allows the amplification of the expression products. Besides the possible use of these proteins in protection experiments, they can also be used as antigens for diagnosis, which opens an efficient approach for control of EBV-related neoplasias by early therapy.

Item Type:

Article

Institutions:

Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene

Identification Number:

Value	Type
2826297	PubMed ID

Classification:

Notation	Type
Animals	MESH
Antigens, Viral/isolation & purification	MESH
Clone Cells/secretion	MESH
Cloning, Molecular	MESH
Cricetinae	MESH
Cricetulus	MESH
Female	MESH
Gene Amplification	MESH
Herpesvirus 4, Human/immunology	MESH
Ovary	MESH
Plasmids	MESH
Viral Matrix Proteins/secretion	MESH
Viral Vaccines/isolation & purification	MESH