Starcich, B. R. and Hahn, B. H. and Shaw, G.M. and McNeely, P. D. and Modrow, Susanne and Wolf, Hans J. and Parks, E. S. and Parks, W. P. and Josephs, S. F. and Gallo, R. C.
Identification and characterization of conserved and variable regions in the envelope gene of HTLV-III/LAV, the retrovirus of AIDS.
Cell 45 (5), pp. 637-648.
To determine the extent and nature of genetic variation present in independent isolates of HTLV-III/LAV, the nucleotide sequences of the entire envelope gene and parts of gag and pol were determined for two AIDS viruses. The results indicated that variation throughout the viral genome is extensive and that the envelope gene in particular is most highly variable. Within the envelope, changes were most prevalent within the extracellular region where clustered nucleotide substitutions and deletions/insertions were evident. Based on predicted secondary protein structure and hydrophilicity, these hypervariable regions represent potential antigenic sites. In contrast to the hypervariable regions, other sequences in the extracellular envelope and the overall envelope structure (including 18 of 18 cysteine residues), as well as most of the transmembrane region, were highly conserved.
|Institutions:|| Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene|
|Acquired Immunodeficiency Syndrome/microbiology||MESH|
|Amino Acid Sequence||MESH|
|Deltaretrovirus/isolation & purification||MESH|
|Gene Products, gag||MESH|
|Nucleic Acid Conformation||MESH|
|RNA-Directed DNA Polymerase/genetics||MESH|
|Sequence Homology, Nucleic Acid||MESH|
|Viral Envelope Proteins/genetics||MESH|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||20 Apr 2011 12:42|
|Last Modified:||21 Jul 2011 02:11|