Hell, W. and Modrow, Susanne and Wolf, Hans J.
Mapping of herpesvirus saimiri proteins on the viral genome: proteins dependent and not dependent on viral DNA synthesis.
Journal of virology 56 (2), pp. 414-418.
Hybrid selected translation was used to map the genome of herpesvirus saimiri, a lymphotropic and oncogenic herpesvirus. RNA extracted from virus-infected cells was hybridized to cloned genomic fragments, and the hybrid selected mRNAs were translated in vitro in a rabbit reticulocyte lysate. Forty-five virus-induced polypeptides were identified and correlated to their coding regions on the herpesvirus saimiri genome. Inhibition of the replication of viral DNA with phosphonoacetic acid showed that 22 of these polypeptides belong to the early group of herpesvirus saimiri gene products.
|Institutions:|| Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene|
|DNA Replication/drug effects||MESH|
|Herpesvirus 2, Saimiriine/genetics||MESH|
|Nucleic Acid Hybridization||MESH|
|Virus Replication/drug effects||MESH|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||20 Apr 2011 12:36|
|Last Modified:||21 Jul 2011 02:12|