Startseite UB

Enhancing the stability and solubility of the glucocorticoid receptor ligand-binding domain by high-throughput library screening

Seitz, Tobias and Thoma, Ralf and Schoch, Guillaume A. and Stihle, Martine and Benz, Jörg and D'Arcy, Brigitte and Wiget, Andrea and Ruf, Armin and Hennig, Michael and Sterner, Reinhard (2010) Enhancing the stability and solubility of the glucocorticoid receptor ligand-binding domain by high-throughput library screening. Journal of molecular biology 403 (4), pp. 562-577.

Full text not available from this repository.

at PubMed

at publisher (via DOI)


Abstract

The human glucocorticoid receptor ligand-binding domain (hGR-LBD) is an important drug target for the treatment of various diseases. However, the low intrinsic stability and solubility of hGR-LBD have rendered its purification and biophysical characterization difficult. In order to overcome these problems, we have stabilized hGR-LBD by a combination of random mutagenesis and high-throughput ...

plus


Export bibliographical data



Item Type:Article
Date:2010
Institutions:Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Reinhard Sterner
Identification Number:
ValueType
20850457PubMed ID
10.1016/j.jmb.2010.08.048DOI
Classification:
NotationType
Amino Acid SequenceMESH
Amino Acid SubstitutionMESH
AnimalsMESH
Base SequenceMESH
Binding Sites/geneticsMESH
Crystallography, X-RayMESH
Escherichia coli/geneticsMESH
Flow CytometryMESH
Gene LibraryMESH
Green Fluorescent Proteins/geneticsMESH
HumansMESH
LigandsMESH
MiceMESH
Models, MolecularMESH
Molecular Sequence DataMESH
MutagenesisMESH
Protein ConformationMESH
Protein EngineeringMESH
Protein StabilityMESH
Protein Structure, TertiaryMESH
Receptors, Glucocorticoid/geneticsMESH
Recombinant Fusion Proteins/geneticsMESH
Sequence Homology, Amino AcidMESH
SolubilityMESH
Static ElectricityMESH
Tumor Necrosis Factor Ligand Superfamily Member 13/geneticsMESH
Subjects:500 Science > 570 Life sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Owner: Universitätsbibliothek Regensburg
Deposited On:03 May 2011 06:01
Last Modified:03 May 2011 06:01
Item ID:20721
Owner Only: item control page
  1. University

University Library

Publication Server

Contact person
Gernot Deinzer

Telefon 0941 943-2759
Contact