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Time-Resolved Luminescence Energy Transfer Immunobinding Study Using a Ruthenium–Ligand Complex as a Donor Label

Augustin, Christine M. and Oswald, Bernhard and Wolfbeis, Otto S. (2002) Time-Resolved Luminescence Energy Transfer Immunobinding Study Using a Ruthenium–Ligand Complex as a Donor Label. Analytical Biochemistry 305 (2), pp. 166-172.

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Abstract

A novel immunosystem is described that exploits the effect of luminescence energy transfer from a luminescently labeled antigen to a fluorescent antibody. A luminescent ruthenium–ligand complex (D-455) with absorption/emission maxima at 456/639 nm, respectively, was employed as the donor label, and a squaraine-type cyanine label (636/655 nm), as the fluorescent acceptor label. Specifically, the ...

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Item Type:Article
Date:2002
Institutions:Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Otto S. Wolfbeis)
Identification Number:
ValueType
10.1006/abio.2002.5633DOI
Keywords:luminescence immunoassay; time resolution; luminescence decay time; resonance energy transfer (RET); fluorescent labels; ruthenium labels
Subjects:500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Owner: Petra Gürster
Deposited On:13 May 2011 08:27
Last Modified:13 Mar 2014 17:19
Item ID:20748
Owner Only: item control page

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