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Red Laser-Induced Fluorescence Energy Transfer in an Immunosystem

Oswald, Bernhard and Lehmann, Frank and Simon, Lydia and Terpetschnig, Ewald and Wolfbeis, Otto S. (2000) Red Laser-Induced Fluorescence Energy Transfer in an Immunosystem. Analytical Biochemistry 280 (2), pp. 272-277.

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Abstract

We describe two near-infrared fluorescent squaraine dyes (Sq635 and Sq660), their spectra, their covalent linkage to proteins, and their use as donor and acceptor, respectively, in a fluorescence resonance energy transfer (FRET) immunoassay based on the use of red lasers. The dyes show quantum yields of around 10% in the free form and up to 68% when bound to proteins. If converted into their ...

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Item Type:Article
Date:2000
Institutions:Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Otto S. Wolfbeis)
Identification Number:
ValueType
10.1006/abio.2000.4553DOI
Subjects:500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Owner: Petra Gürster
Deposited On:13 May 2011 08:47
Last Modified:13 Mar 2014 17:20
Item ID:20750
Owner Only: item control page
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