Wolf, Hans J. and zur Hausen, H. and Klein, G. and Becker, V. and Henle, G. and Henle, W.
Attempts to detect virus-specific DNA sequences in human tumors. III. Epstein-Barr viral DNA in non-lymphoid nasopharyngeal carcinoma cells.
Medical microbiology and immunology 161 (1), pp. 15-21.
Fourteen tumors of the nasopharyngeal region were analyzed for the presence of Epstein-Barr virus-specific DNA by DNA-cRNA hybridization. These data were compared to the histology of the respective tumors and the seroreactivity of the tumor-bearing patient against EBV-related antigens. With one exception, all tumor pieces containing nasopharyngeal carcinoma cells hybridized significantly with EBV-cRNA. Tumors of predominantly epithelial morphology annealed in the highest range. In situ-hybridization of freeze sections from a tumor containing almost equal amounts of tumor cells and lymphocytes revealed hybridizing DNA within nuclei of non-lymphoid cells. Although these data do not exclude the presence of EBV-DNA within lymphoid cells, they clearly demonstrate that in nasopharyngeal carcinomas the vast majority of EBV-specific DNA rests within non-lymphoid cells.
|Institutions:|| Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene|
|Carcinoma, Squamous Cell/microbiology||MESH|
|DNA, Neoplasm/isolation & purification||MESH|
|DNA, Viral/isolation & purification||MESH|
|Herpesvirus 4, Human/isolation & purification||MESH|
|Nucleic Acid Hybridization||MESH|
|RNA, Neoplasm/isolation & purification||MESH|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||05 May 2011 06:35|
|Last Modified:||21 Jul 2011 02:12|