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Capacitive monitoring of protein immobilization and antigen–antibody reactions on monomolecular alkylthiol films on gold electrodes

Mirsky, Vladimir M. and Riepl, Michael and Wolfbeis, Otto S. (1997) Capacitive monitoring of protein immobilization and antigen–antibody reactions on monomolecular alkylthiol films on gold electrodes. Biosensors and Bioelectronics 12 (9-10), pp. 977-989.

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Abstract

Self-assembled monolayers of v-mercaptohexadecanoic acid and vmercaptohexadecylamine on gold electrodes are stable at neutral pH and display pure capacitive behavior at frequencies around 20 Hz. Different methods of covalent immobilization of proteins on these monolayers are compared. Various reagents including succinimides, thionylchloride, p-nitrophenol and carbodiimides were used to activate ...

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Item Type:Article
Date:1997
Institutions:Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Otto S. Wolfbeis)
Keywords:protein immobilization, self-assembly, immunosensor, impedance, capacitive sensors
Subjects:500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Owner: Petra Gürster
Deposited On:06 Jun 2011 13:48
Last Modified:13 Mar 2014 17:27
Item ID:21088
Owner Only: item control page

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