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Antigen-specific electrophoretic cell separation (ASECS): isolation by human T and B lymphocyte subpopulations by free-flow electrophoresis after reaction with antibodies

Hansen, Ernil and Hannig, Kurt (1982) Antigen-specific electrophoretic cell separation (ASECS): isolation by human T and B lymphocyte subpopulations by free-flow electrophoresis after reaction with antibodies. Journal of immunological methods 51 (2), pp. 197-208.

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Abstract

The electrophoretic mobility of human lymphocytes can be reduced by incubation with surface antigen specific antibodies under non-capping conditions. This renders subpopulations of human peripheral blood lymphocytes accessible to separation by free-flow electrophoresis. After reaction of lymphocyte preparations with anti-IgM antibody and a fluorescent second antibody, B lymphocytes showed a ...

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Item Type:Article
Date:1982
Institutions:Medicine > Lehrstuhl für Anästhesiologie
Identification Number:
ValueType
6180026PubMed ID
Classification:
NotationType
AnimalsMESH
Antibodies, Anti-IdiotypicMESH
Antibodies, Monoclonal/immunologyMESH
Antigen-Antibody ReactionsMESH
B-LymphocytesMESH
Cell MovementMESH
Cell Separation/methodsMESH
Electrophoresis/methodsMESH
EpitopesMESH
Flow CytometryMESH
GoatsMESH
HumansMESH
Immunoglobulin MMESH
MiceMESH
RabbitsMESH
RatsMESH
T-LymphocytesMESH
Subjects:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Unknown
Owner: Universitätsbibliothek Regensburg
Deposited On:05 Sep 2011 13:33
Last Modified:13 Mar 2014 17:55
Item ID:21989
Owner Only: item control page
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