Blaschek, W. and Koehler, H. and Semler, U. and Franz, Gerhard (1982) Molecular weight distribution of cellulose in primary cell walls. Planta 154, pp. 550-555.
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The distribution pattern of the degree of polymerization (DP) of cellulose present in the cell walls of mesophyll- and suspension-cultured cells of tobacco was compared to that of newly synthesized 14C-labeled cellulose from regenerating tobacco protoplasts and suspension-cultured cells. The cellulose was nitrated, and, after fractionation according to differences in solubility in acetone/water, the DP pattern of labeled or unlabeled cellulose nitrate was determined by viscosity measurements. A low (DP<500) and="" high="" dp-fraction="" (dp="">2500) of cellulose were predominant in the cell walls of protoplasts, suspension — cultured cells, and mesophyll cells. The average DP of the high molecular weight fraction of cellulose in the cell walls of mesophyll was higher (DPsim4,000) than in protoplasts or suspension — cultured cells (DP 2,500-3,000). In all cell walls tested, minor amounts of cellulose molecules with a broad spectrum of a medium DP were present. Pulse — chase experiments with either protoplasts or suspension —cultured cells showed that a large proportion of the low and medium DP-cellulose are a separate class of structural components of the cellulose network. The results are discussed in relation to the organization of cellulose in the primary cell wall.
|Institutions:||Chemistry and Pharmacy > Institute of Pharmacy > Retired Professors > Prof. Franz|
|Keywords:||Cell culture - Cell wall, primary cellulose (polymerization) - Mesophyll - Nicotiana (cell wall) - Protoplast|
|Subjects:||500 Science > 540 Chemistry & allied sciences|
500 Science > 570 Life sciences
|Created at the University of Regensburg:||Unknown|
|Deposited On:||15 Nov 2011 07:31|
|Last Modified:||15 Nov 2011 07:31|