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Generation of specific antibodies against the rap1A, rap1B and rap2 small GTP-binding proteins. Analysis of rap and ras proteins in membranes from mammalian cells

Klinz, F. J. and Seifert, Roland and Schwaner, I. and Gausepohl, H. and Frank, R. and Schultz, Günter (1992) Generation of specific antibodies against the rap1A, rap1B and rap2 small GTP-binding proteins. Analysis of rap and ras proteins in membranes from mammalian cells. European journal of biochemistry 207 (1), pp. 207-213.

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Abstract

Specific antibodies against rap1A and rap1B small GTP-binding proteins were generated by immunization of rabbits with peptides derived from the C-terminus of the processed proteins. Immunoblot analysis of membranes from several mammalian cell lines and human thrombocytes with affinity-purified antibodies against rap1A or rap1B demonstrated the presence of multiple immunoreactive proteins in the ...

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Item Type:Article
Date:1992
Institutions:Chemistry and Pharmacy > Institute of Pharmacy > Pharmacology and Toxicology (Prof. Schlossmann formerly Prof. Seifert)
Identification Number:
ValueType
1628649PubMed ID
Classification:
NotationType
Amino Acid SequenceMESH
AnimalsMESH
AntibodiesMESH
Antibody SpecificityMESH
Blood Platelets/chemistryMESH
Cell LineMESH
Cell Membrane/chemistryMESH
Cytosol/chemistryMESH
Electrophoresis, Polyacrylamide GelMESH
Escherichia coli/geneticsMESH
GTP-Binding Proteins/immunologyMESH
HumansMESH
ImmunoblottingMESH
Molecular Sequence DataMESH
Molecular WeightMESH
Proto-Oncogene Proteins/analysisMESH
Proto-Oncogene Proteins p21(ras)/geneticsMESH
Rabbits/immunologyMESH
Recombinant Proteins/immunologyMESH
Sequence Homology, Nucleic AcidMESH
rap GTP-Binding ProteinsMESH
Subjects:600 Technology > 615 Pharmacy
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Unknown
Owner: Universitätsbibliothek Regensburg
Deposited On:24 Jan 2012 07:57
Last Modified:13 Mar 2014 18:28
Item ID:23220
Owner Only: item control page
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