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G-protein alpha-subunits in cytosolic and membranous fractions of human neutrophils

Rudolph, U. and Koesling, D. and Hinsch, K. D. and Seifert, Roland and Bigalke, M. and Schultz, Günter and Rosenthal, W. (1989) G-protein alpha-subunits in cytosolic and membranous fractions of human neutrophils. Molecular and cellular endocrinology 63 (1-2), pp. 143-153.

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Abstract

In plasma membranes of human neutrophils, we identified two major pertussis toxin substrates of 40 kDa Mr with pI values of 5.30 and 5.37. Only the acidic of the two substrates was also present in neutrophil cytosol. Two-dimensional tryptic peptide maps revealed a high degree of homology of cytosolic and particulate substrates. Purified G-protein beta gamma-complex stimulated pertussis ...

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Item Type:Article
Date:1989
Institutions:Chemistry and Pharmacy > Institute of Pharmacy > Pharmacology and Toxicology (Prof. Schlossmann formerly Prof. Seifert)
Identification Number:
ValueType
2502457PubMed ID
Classification:
NotationType
Cell Membrane/ultrastructureMESH
Centrifugation, Density GradientMESH
Cytosol/analysisMESH
Electrophoresis, Polyacrylamide GelMESH
GTP-Binding Proteins/analysisMESH
HumansMESH
ImmunoblottingMESH
Neutrophils/ultrastructureMESH
Subjects:600 Technology > 610 Medical sciences Medicine
600 Technology > 615 Pharmacy
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Unknown
Owner: Universitätsbibliothek Regensburg
Deposited On:24 Jan 2012 11:52
Last Modified:13 Mar 2014 18:29
Item ID:23254
Owner Only: item control page
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