Seifert, Roland and Schultz, Günter
Reversible activation of NADPH oxidase in membranes of HL-60 human leukemic cells.
Biochemical and biophysical research communications 146 (3), pp. 1296-1302.
NADPH oxidase in membranes of undifferentiated and dimethylsulphoxide-differentiated HL-60 cells was activated by arachidonic acid (AA) in the presence of Mg2+ and a cytosolic cofactor (CF) found in differentiated HL-60 cells. Basal superoxide (O2-) formation was enhanced several-fold by addition of the stable GTP-analogue, guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S), prior to AA and was ...
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