Müller, Rainer and Abke, Jochen and Macionczyk, U. and Gbureck, U. and Ruszczak, Z. and Mehrl, R. and Kujat, R. and Englert, Carsten and Nerlich, Michael and Angele, Peter (2005) Surface engineering of stainless steel materials by covalent collagen immobilization to improve implant biocompatibility. Biomaterials 26 (34), pp. 6962-6972.
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It was shown recently that the deposition of thin films of tantalum and tantalum oxide enhanced the long-term biocompatibility of stainless steel biomaterials due to an increase in their corrosion resistance. In this study, the authors used this tantalum oxide coating as a basis for covalent immobilization of a collagen layer, which should result in a further improvement of implant tissue integration. Because of the high degrdn. rate of natural collagen in vivo, covalent immobilization as well as carbodiimide induced crosslinking of the protein was performed. It was found that the combination of the silane-coupling agent aminopropyl triethoxysilane and the linker mol. N,N'-disulfosuccinimidyl suberate was a very effective system for collagen immobilizing. Mech. and enzymic stability testing revealed a higher stability of covalent bound collagen layers compared to phys. adsorbed collagen layers. The biol. response induced by the surface modifications was evaluated by in vitro cell culture with human mesenchymal stem cells as well as by in vivo s.c. implantation into nude mice. The presence of collagen clearly improved the cytocompatibility of the stainless steel implants which, nevertheless, significantly depended on the crosslinking degree of the collagen layer.
|Institutions:||Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry VI - Physical Chemistry (Solution Chemistry) > Prof. Dr. Werner Kunz|
|Keywords:||stainless steel implant surface modification collagen crosslinking stem cell|
|Subjects:||500 Science > 540 Chemistry & allied sciences|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Yes|
|Deposited On:||19 Jun 2012 06:04|
|Last Modified:||19 Jun 2012 06:04|