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Local Tertiary Structure Probing of Ribonucleoprotein Particles by Nuclease Fusion Proteins

Ohmayer, Uli and Perez-Fernandez, Jorge and Hierlmeier, Thomas and Pöll, Gisela and Williams, Lydia and Griesenbeck, Joachim and Tschochner, Herbert and Milkereit, Philipp (2012) Local Tertiary Structure Probing of Ribonucleoprotein Particles by Nuclease Fusion Proteins. Plos One 7, e42449.

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Abstract

Analyses of the conformational dynamics of the numerous cellular ribonucleoprotein particles (RNP) significantly contribute to the understanding of their modes of action. Here, we tested whether ribonuclease fusion proteins incorporated into RNPs can be used as molecular probes to characterize the local RNA environment of these proteins. Fusion proteins of micrococcal nuclease (MNase) with ...

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Item Type:Article
Date:2 August 2012
Institutions:Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Herbert Tschochner
Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Dr. Joachim Griesenbeck
Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Dr. Philipp Milkereit
Projects:Open Access Publizieren (DFG)
Identification Number:
ValueType
doi:10.1371/journal.pone.0042449DOI
Subjects:500 Science > 570 Life sciences
600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Owner: Universitätsbibliothek Regensburg
Deposited On:07 Sep 2012 06:58
Last Modified:13 Mar 2014 19:12
Item ID:25848
Owner Only: item control page

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