Schmid, Peter M. and Heid, Iris M. and Buechler, Christa and Steege, Andreas and Resch, Markus and Birner, Christoph and Endemann, Dierk and Riegger, Günter A. J. and Luchner, Andreas (2012) Expression of fourteen novel obesity-related genes in zucker diabetic fatty rats. Cardio Vascular Diabetology 11, p. 48.
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Genome-wide association studies (GWAS) are useful to reveal an association between single nucleotide polymorphisms and different measures of obesity. A multitude of new loci has recently been reported, but the exact function of most of the according genes is not known. The aim of our study was to start elucidating the function of some of these genes.
We performed an expression analysis of fourteen genes, namely BDNF, ETV5, FAIM2, FTO, GNPDA2, KCTD15, LYPLAL1, MCR4, MTCH2, NEGR1, NRXN3, TMEM18, SEC16B and TFAP2B, via real-time RT-PCR in adipose tissue of the kidney capsule, the mesenterium and subcutaneum as well as the hypothalamus of obese Zucker diabetic fatty (ZDF) and Zucker lean (ZL) rats at an age of 22 weeks.
All of our target genes except for SEC16B showed the highest expression in the hypothalamus. This suggests a critical role of these obesity-related genes in the central regulation of energy balance. Interestingly, the expression pattern in the hypothalamus showed no differences between obese ZDF and lean ZL rats. However, LYPLAL1, TFAP2B, SEC16B and FAIM2 were significantly lower expressed in the kidney fat of ZDF than ZL rats. NEGR1 was even lower expressed in subcutaneous and mesenterial fat, while MTCH2 was higher expressed in the subcutaneous and mesenterial fat of ZDF rats.
The expression pattern of the investigated obesity genes implies for most of them a role in the central regulation of energy balance, but for some also a role in the adipose tissue itself. For the development of the ZDF phenotype peripheral rather than central mechanisms of the investigated genes seem to be relevant.
|Date:||3 May 2012|
|Institutions:|| Medicine > Lehrstuhl für Innere Medizin I|
Medicine > Lehrstuhl für Innere Medizin II
Medicine > Institut für Epidemiologie und Präventivmedizin
|Projects:||Open Access Publizieren (DFG)|
|Keywords:||BDNF; ETV5; FAIM2; FTO; GNPDA2; KCTD15; LYPLAL1; MCR4; MTCH2; NEGR1; NRXN3; TMEM18; SEC16B; TFAP2B; ZDF-rats|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Yes|
|Deposited On:||24 Oct 2012 10:27|
|Last Modified:||21 Mar 2013 09:55|