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Qualitative and quantitative analysis of hyaluronan oligosaccharides with high performance thin layer chromatography using reagent-free derivatization on amino-modified silica and electrospray ionization-quadrupole time-of-flight mass spectrometry coupling on normal phase.

Rothenhöfer, Martin and Scherübl, Rosmarie and Bernhardt, Günther and Heilmann, Jörg and Buschauer, Armin (2012) Qualitative and quantitative analysis of hyaluronan oligosaccharides with high performance thin layer chromatography using reagent-free derivatization on amino-modified silica and electrospray ionization-quadrupole time-of-flight mass spectrometry coupling on normal phase. Journal of chromatography. A 1248, pp. 169-77.

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Abstract

Purified oligomers of hyalobiuronic acid are indispensable tools to elucidate the physiological and pathophysiological role of hyaluronan degradation by various hyaluronidase isoenzymes. Therefore, we established and validated a novel sensitive, convenient, rapid, and cost-effective high performance thin layer chromatography (HPTLC) method for the qualitative and quantitative analysis of small ...

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Item Type:Article
Date:27 July 2012
Institutions:Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical/Medicinal Chemistry II (Prof. Buschauer)
Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Biology (Prof. Heilmann)
Identification Number:
ValueType
22721763PubMed ID
10.1016/j.chroma.2012.05.057DOI
Classification:
NotationType
Amines/chemistryMESH
CalibrationMESH
Chromatography, Thin Layer/methodsMESH
Densitometry/methodsMESH
Hyaluronic Acid/isolation & purificationMESH
Limit of DetectionMESH
Oligosaccharides/analysisMESH
Silicon Dioxide/chemistryMESH
Spectrometry, Mass, Electrospray Ionization/methodsMESH
Streptococcus/chemistryMESH
Keywords:Hyalobiuronic acid; Hyaluronan; HPTLC; Amino-modified silica; Densitometric quantification
Subjects:500 Science > 540 Chemistry & allied sciences
500 Science > 570 Life sciences
600 Technology > 615 Pharmacy
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Owner: Prof. Armin Buschauer
Deposited On:05 Nov 2012 06:02
Last Modified:05 Nov 2012 06:02
Item ID:26584
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