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Monitoring the Degradation of Reduction-Sensitive Gene Carriers with Fluorescence Spectroscopy and Flow Cytometry

Hozsa, Constantin ; Breunig, Miriam ; Goepferich, Achim



Zusammenfassung

Polycations like poly(ethylene imine) (PEI) or poly(l-lysine) (pLL) form nanometer-sized complexes with nucleic acids (polyplexes) which can be used for gene delivery. It is known that the properties of these ­carriers can be greatly improved by introducing disulfide bridges on the polymers, thus making them reduction sensitive. However, little is known about how such modified carriers behave ...

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