Zusammenfassung
Blue light sensors using FAD (BLUF) domains are flavin based blue light photoreceptors. The BLUF domains are often fused with various effector domains. BLUF domain coupled with adenylyl cyclase domain is known as photoactivated adenylyl cyclase (PAC). Naegleria gruberi genome database analysis revealed the presence of four PACs. Each of the photoactivated adenylyl cyclases from Naegleria gruberi ...
Zusammenfassung
Blue light sensors using FAD (BLUF) domains are flavin based blue light photoreceptors. The BLUF domains are often fused with various effector domains. BLUF domain coupled with adenylyl cyclase domain is known as photoactivated adenylyl cyclase (PAC). Naegleria gruberi genome database analysis revealed the presence of four PACs. Each of the photoactivated adenylyl cyclases from Naegleria gruberi (NgPACs) is composed of a BLUF domain and an adenylyl cyclase domain. Light regulated enzymatic activity of recombinant NgPAC1 protein was assayed in dark and after blue light irradiation by measuring the cAMP level. Experimental results showed that the NgPAC1 protein exhibits light regulated cyclase activity. In this report, we have also demonstrated that the recombinant NgPAC1 exits as an oligomer in solution.
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