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Determination of affinity and activity of ligands at the human neuropeptide Y Y4 receptor by flow cytometry and aequorin luminescence.

Ziemek, Ralf and Schneider, Erich and Kraus, Anja and Cabrele, Chiara and Beck-Sickinger, Annette G. and Bernhardt, Günther and Buschauer, Armin (2007) Determination of affinity and activity of ligands at the human neuropeptide Y Y4 receptor by flow cytometry and aequorin luminescence. Journal of Receptors and Signal Transduction 27 (4), pp. 217-233.

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Other URL: http://www.informaworld.com/smpp/content?content=10.1080/10799890701505206


Abstract

Fluorescence-labeled neuropeptide Y (NPY) has been used in flow cytometric binding assays for the determination of affinity constants of NPY Y1, Y2, and Y5 receptor ligands. Because the binding of fluorescent NPY is insufficient for competition studies at the human Y4 receptor (hY4R), we replaced Glu-4 in hPP with Lys for the derivatization with cyanine-5. Because cy5-[K(4)]hPP has high affinity ...

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Item Type:Article
Date:2007
Institutions:Chemistry and Pharmacy > Institut für Organische Chemie > Arbeitskreis Dr. Chiara Cabrele
Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical/Medicinal Chemistry II (Prof. Buschauer)
Projects:GRK 760, Graduiertenkolleg Medizinische Chemie
Identification Number:
ValueType
10.1080/10799890701505206DOI
17885919PubMed ID
Keywords:NPY Y4 receptor; Flow cytometry; Chimeric G protein; Aequorin; Nonpeptide NPY Y4 receptor antagonist
Subjects:500 Science > 570 Life sciences
600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Owner: Prof. Armin Buschauer
Deposited On:21 Feb 2008 08:05
Last Modified:05 Aug 2009 13:42
Item ID:3378
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