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A Simple and Powerful Flow Cytometric Method for the Simultaneous Determination of Multiple Parameters at G-Protein-coupled Receptor Subtypes.

Schneider, Erich and Mayer, Matthias and Ziemek, Ralf and Li, Liantao and Hutzler, Christoph and Bernhardt, Günther and Buschauer, Armin (2006) A Simple and Powerful Flow Cytometric Method for the Simultaneous Determination of Multiple Parameters at G-Protein-coupled Receptor Subtypes. ChemBioChem 7 (9), pp. 1400-1409.

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Other URL: http://www3.interscience.wiley.com/cgi-bin/abstract/112746899/ABSTRACT


Abstract

The quantification of pharmacological parameters at G protein-coupled receptors (GPCRs) is indispensable in drug research but costly and time-consuming when conventional methods are sequentially applied. With neuropeptide Y (NPY) Y(1), Y(2), and Y(5) receptors as model systems, a homogenous flow cytometric method for the simultaneous determination of the affinity, selectivity, and activity of ...

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Item Type:Article
Date:2006
Institutions:Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical/Medicinal Chemistry II (Prof. Buschauer)
Projects:GRK 760, Graduiertenkolleg Medizinische Chemie
Identification Number:
ValueType
10.1002/cbic.200600163DOI
16888730PubMed ID
Keywords:binding affinity; calcium mobilization; flow cytometry; fluorescent probes; receptors
Subjects:500 Science > 570 Life sciences
600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Owner: Prof. Armin Buschauer
Deposited On:21 Feb 2008 08:35
Last Modified:05 Aug 2009 13:42
Item ID:3385
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