Oettl, Martin and Hoechstetter, Julia and Asen, Iris and Bernhardt, Günther and Buschauer, Armin (2003) Comparative characterization of bovine testicular hyaluronidase and a hyaluronate lyase from Streptococcus agalactiae in pharmaceutical preparations. European Journal of Pharmaceutical Sciences 18 (3-4), pp. 267-277.
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Although bovine testicular hyaluronidase (BTH) has been used in several medical fields for many years, these drugs are poorly characterized. We compared pharmaceutical BTH preparations (Neopermease®, Hylase® “Dessau”) and a hyaluronate lyase from Streptococcus agalactiae. The BTH preparations were complex mixtures of proteins (SDS–PAGE, gel filtration) with enzymatic activity in different fractions. In the case of Neopermease® the highest specific activity was found in the 58 kDa fraction (optimum at pH 3.6), whereas the 77 and 33 kDa fractions showed markedly lower specific activities at an optimal pH of 6.2. Maximum specific activity of the bacterial enzyme (approx. 1000 μmol min−1 mg−1) was found at pH 5.0, being 410- and 5100-times higher compared to Neopermease® and Hylase® “Dessau”, respectively. The hyaluronate lyase preparation was separated into two main proteins [100 kDa (pI=8.9) and 85 kDa (pI=9.2)] which were enzymatically active in SDS substrate–PAGE. Zymography after limited proteolysis of the bacterial enzyme with trypsin revealed active fragments (75–50 kDa). Our results suggest that hyaluronate lyase is an alternative for BTH, of which there has been a shortage, since companies have stopped the production of BTH preparations due to the risk of BSE.
|Institutions:||Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical/Medicinal Chemistry II (Prof. Buschauer)|
|Keywords:||Bovine testicular hyaluronidase; Hyaluronate lyase; Streptococcus agalactiae; SDS–PAGE; Zymography; Limited proteolysis|
|Subjects:||500 Science > 570 Life sciences|
600 Technology > 610 Medical sciences Medicine
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Yes|
|Owner:||Prof. Armin Buschauer|
|Deposited On:||21 Feb 2008 10:19|
|Last Modified:||05 Aug 2009 13:42|