Guo, Huimin and Kottke, Tilman and Hegemann, Peter and Dick, Bernhard (2005) The phot LOV2 domain and its interaction with LOV1. Biophysical Journal 89 (1), pp. 402-412.
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Phot proteins are homologs of the blue-light receptor phototropin. We report a comparative study of the photocycles of the isolated, light-sensitive domains LOV1 and LOV2 from Chlamydomonas reinhardtii phot protein, as well as the construct LOV1/2 contg. both domains. Transient absorption measurements revealed a short lifetime of the LOV2-wt triplet state (500 ns), but a long lifetime (287 ms) of the triplet in the mutant LOV2-C250S, in which the reactive cysteine is replaced by serine. For LOV1, in comparison, corresponding nos. of 800 ns and 4 ms for the two conformers in LOV1-wt, and 27 ms for LOV1-C57S have been reported. The triplet decay kinetics in the mixed domains LOV1/2-wt, LOV1/2-C57S, and LOV1/2-C250S can be analyzed as the superposition of the behavior of the corresponding single domains. The situation is different for the slow, thermal reaction of the photoadduct back to the dark form. Whereas the individual domains LOV1 and LOV2 show two decay components, the double domains LOV1/2-C57S and LOV1/2-C250S both show only a single component. The interaction of the two domains does therefore not manifest itself during the lifetime of the triplet states, but changes the decay behavior of the adduct states.
|Additional information (public):||CAN 143:224542 6-3 General Biochemistry|
|Institutions:||Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry III - Physical Chemistry (Light and Matter) > Prof. Dr. Bernhard Dick|
|Projects:||GRK 640 Sensory photoreceptors in natural and artificial systems, Blaulicht-sensitive Photorezeptoren|
|Keywords:||Protein motifs (LOV1, phot LOV2 domain and its interaction with LOV1); Protein motifs (LOV2, phot LOV2 domain and its interaction with LOV1); Chlamydomonas reinhardtii (phot LOV2 domain and its interaction with LOV1); Phototropins, Role: BSU (Biological study, unclassified), PRP (Properties), BIOL (Biological study) (phot LOV2 domain and its interaction with LOV1); phototropin LOV2 domain interaction LOV1 photocycle|
|Subjects:||500 Science > 570 Life sciences|
500 Science > 540 Chemistry & allied sciences
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Yes|
|Owner:||Prof. Dr. Bernhard Dick|
|Deposited On:||15 Jan 2009 16:55|
|Last Modified:||31 Aug 2012 09:15|