Kottke, Tilman and Dick, Bernhard and Fedorov, Roman and Schlichting, Ilme and Deutzmann, Rainer and Hegemann, Peter (2003) Irreversible Photoreduction of Flavin in a Mutated Phot-LOV1 Domain. Biochemistry 42 (33), pp. 9854-9862.
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Phot photoreceptors make up an important protein family regulating biol. processes in response to blue light. They contain two light, oxygen, and voltage sensitive (LOV) domains and a serine/threonine kinase domain. Both LOV domains noncovalently bind a FMN. Upon absorption of blue light, the LOV domains undergo a photocycle, transiently forming a covalent adduct of a cysteine residue and the FMN (LOV-390). The mechanism of formation of this flavin-thiol adduct is still unclear. The authors studied a mutant of the LOV1 domain from the green alga Chlamydomonas reinhardtii with a methionine replacing the reactive cysteine 57 (C57M). As in the wild type, irradn. leads to formation of a photoadduct, which, however, is irreversibly converted into a red absorbing species, C57M-675. On the basis of spectroscopic results and the 2.1 .ANG. resoln. crystal structure, this highly unusual FMN species was assigned to a neutral flavin radical covalently attached to the apoprotein at the N(5) position. In contrast to other flavoprotein neutral radicals, C57M-675 is stable even under aerobic or denaturing conditions. Pathways for the photoinduced formation of the adduct are discussed for the C57M mutant as well as the wild-type LOV1 domain.
|Additional information (public):||CAN 139:256942 6-3 General Biochemistry 146-17-8D (FMN) Role: PEP (Physical, engineering or chemical process), PRP (Properties), PYP (Physical process), PROC (Process) (irreversible photoredn. of FMN in Phot photoreceptor LOV1 domain mutant)|
|Institutions:||Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry III - Physical Chemistry (Light and Matter) > Prof. Dr. Bernhard Dick|
|Projects:||GRK 640 Sensory photoreceptors in natural and artificial systems|
|Keywords:||Protein motifs (LOV (light, oxygen and voltage sensitive) domain,irreversible photoredn. of FMN in Phot photoreceptor LOV1 domain mutant); Phototropins, Role: PEP (Physical, engineering or chemical process), PRP (Properties), PYP (Physical process), PROC (Process) (LOV1 domain, C57M mutant irreversible photoredn. of FMN in Phot photoreceptor LOV1 domain mutant); Reduction, photochemical (irreversible photoredn. of FMN in Phot photoreceptor LOV1 domain mutant); Crystal structure; Total energy (of FMN photoadduct with Phot photoreceptor LOV1 domain mutant); Phot photoreceptor LOV1 domain FMN photoadduct|
|Subjects:||500 Science > 570 Life sciences|
500 Science > 540 Chemistry & allied sciences
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Yes|
|Owner:||Prof. Dr. Bernhard Dick|
|Deposited On:||15 Jan 2009 17:53|
|Last Modified:||31 Aug 2012 11:16|
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