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Hofmeister specific-ion effects on enzyme activity and buffer pH: Horseradish peroxidase in citrate buffer

Bauduin, Pierre and Nohmie, Fawaz and Touraud, Didier and Neueder, Roland and Kunz, Werner and Ninham, Barry W. (2006) Hofmeister specific-ion effects on enzyme activity and buffer pH: Horseradish peroxidase in citrate buffer. Journal of Molecular Liquids 123 (1), pp. 14-19.

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Other URL: http://dx.doi.org/10.1016/j.molliq.2005.03.003


Abstract

Salt addition to enzymes in buffer always induce the problem of the respective influences of electrostatic interactions and anion specificity on buffer pH and enzyme kinetics. In the present paper the influence of some sodium salts (Na2SO4, NaCl, NaBr and NaNO3) on the pH of a citrate buffer (c = 0.025 M), and on the catalytic constants of horseradish peroxidase (HRP) is studied. First, the pH ...

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Item Type:Article
Date:15 January 2006
Institutions:Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie
Identification Number:
ValueType
10.1016/j.molliq.2005.03.003DOI
Keywords:Hofmeister effects; Ion specificity; Horseradish peroxidase; Enzyme kinetics; Electrostatics
Subjects:500 Science > 530 Physics
500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Unknown
Owner: Ute Lange
Deposited On:30 Jun 2006
Last Modified:13 Mar 2014 09:37
Item ID:8
Owner Only: item control page

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