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Single Molecule Fluorescence Spectroscopy at Ambient Temperature: Design and Application of a Time-Resolved Confocal Microscope with Single-Molecule Sensitivity

URN to cite this document: urn:nbn:de:bvb:355-opus-724

Böhmer, Martin (2002) Single Molecule Fluorescence Spectroscopy at Ambient Temperature: Design and Application of a Time-Resolved Confocal Microscope with Single-Molecule Sensitivity. PhD, Universität Regensburg

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Abstract (German)

Diese Dissertation beschreibt die Entwicklung und Konstruktion eines zeitaufgelösten konfokalen Laser-Scanning Mikroskops für die hoch sensitive Fluoreszenzdetektion in Lösung und auf Oberflächen. Mit diesem Mikroskop lassen sich einzelne, fluoreszierende Moleküle in Lösungen und auf Oberflächen detektieren.

Translation of the abstract (English)

This thesis describes the development and construction of a time-resolved confocal laser-scanning microscope for ultrasensive fluorescence detection in solution and on surfaces. The system is capable of monitoring single molecules in liquids and on surfaces, capable of performing emission and/or polarization spectroscopy, time-resolved spectroscopy with picosecond temporal resolution, and fluorescence correlation spectroscopy with sub-microsecond temporal resolution. Moreover, it allows ultrafast scanning of surfaces thus allowing wavelength, polarization and lifetime imaging of immobilized molecules and samples. Thus, several novel theoretical concepts of single molecule spectroscopy could be experimentally realized and tested.

Item Type:Thesis of the University of Regensburg (PhD)
Referee:Jörg Enderlein
Date of exam:04 February 2002
Institutions: Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik
Keywords:Fluoreszenz , Spektroskopie , Mikroskopie , Einzelmolekülspektroskopie , Single Molecule Spectroscopy
Subjects:500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Owner:Universitätsbibliothek Regensburg
Deposited On:21 Oct 2009 15:41
Last Modified:14 Nov 2012 07:16
Item ID:9917
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