Photocontrolling the Enantioselectivity of a Phosphotriesterase via Incorporation of a Light-Responsive Unnatural Amino Acid

URN to cite this document:: urn:nbn:de:bvb:355-epub-752481
DOI to cite this document:: 10.5283/epub.75248

Hiefinger, Caroline ; Zinner, Gabriel ; Fürtges, Torben F. ; Narindoshvili, Tamari ; Schindler, Sebastian ; Bruckmann, Astrid

; Rudack, Till ; Raushel, Frank M.

; Sterner, Reinhard

License: Creative Commons Attribution 4.0
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Date of publication of this fulltext: 14 Mar 2025 09:54

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Abstract

The external control of catalytic activity and substrate specificity of enzymes by light has aroused great interest in the fields of biocatalysis and pharmacology. Going beyond, we have attempted to photocontrol enzyme stereoselectivity on the example of phosphotriesterase (PTE), which is capable of hydrolyzing a wide variety of racemic organophosphorus substrates where one of two enantiomers is ...