@article{epub57433,
       publisher = {Elsevier},
         address = {AMSTERDAM},
            year = {2022},
          author = {Wiebke Maurer and Nico Hartmann and Loukas Argyriou and Samuel Sossalla and Katrin Streckfuss-B{\"o}meke},
           pages = {102677},
          volume = {60},
         journal = {Stem Cell Research},
           title = {Generation of homozygous Nav1.8 knock-out iPSC lines by CRISPR Cas9 genome editing to investigate a potential new antiarrhythmic strategy},
             url = {https://epub.uni-regensburg.de/57433/},
        abstract = {The sodium channel Na(v)1.8, encoded by SCN10A, is reported to contribute to arrhythmogenesis by inducing the late INa and thereby enhanced persistent Na+ current. However, its exact electrophysiological role in cardiomyocytes remains unclear. Here, we generated induced pluripotent stem cells (iPSCs) with a homozygous SCN10A knock-out from a healthy iPSC line by CRISPR Cas9 genome editing. The edited iPSCs maintained full pluripotency, genomic integrity, and spontaneous in vitro differentiation capacity. The iPSCs are able to differentiate into iPSC-cardiomyocytes, hence making it possible to investigate the role of Na(v)1.8 in the heart.}
}