eprintid: 57433
rev_number: 1
eprint_status: archive
userid: 3883
dir: documents_old/00/05/74/33
datestamp: 2024-02-29 12:56:06
lastmod: 2024-02-29 12:56:06
status_changed: 2024-02-29 12:56:06
type: article
metadata_visibility: show
contact_email: katrin.streckfuss-boemeke@uni-wuerzburg.de
creators_name: Maurer, Wiebke
creators_name: Hartmann, Nico
creators_name: Argyriou, Loukas
creators_name: Sossalla, Samuel
creators_name: Streckfuss-Bömeke, Katrin
title: Generation of homozygous Nav1.8 knock-out iPSC lines by CRISPR Cas9 genome editing to investigate a potential new antiarrhythmic strategy
ispublished: pub
subjects: ddc_2_610
institutions: fak04_16
full_text_status: none
abstract: The sodium channel Na(v)1.8, encoded by SCN10A, is reported to contribute to arrhythmogenesis by inducing the late INa and thereby enhanced persistent Na+ current. However, its exact electrophysiological role in cardiomyocytes remains unclear. Here, we generated induced pluripotent stem cells (iPSCs) with a homozygous SCN10A knock-out from a healthy iPSC line by CRISPR Cas9 genome editing. The edited iPSCs maintained full pluripotency, genomic integrity, and spontaneous in vitro differentiation capacity. The iPSCs are able to differentiate into iPSC-cardiomyocytes, hence making it possible to investigate the role of Na(v)1.8 in the heart.
date_type: published
date_online: 2022
date: 2022
publication: Stem Cell Research
volume: 60
publisher: Elsevier
place_of_pub: AMSTERDAM
pagerange: 102677
article_number: 102677
id_number_name: 10.1016/j.scr.2022.102677
id_number_type: doi
refereed: yes
created_here: yes
issn: 1873-5061
issn:  1876-7753
official_url: http://doi.org/10.1016/j.scr.2022.102677
referencetext: Ahmad S, 2019, ESC HEART FAIL, V6, P154, DOI 10.1002/ehf2.12378
 Bengel P, 2021, NAT COMMUN, V12, DOI 10.1038/s41467-021-26690-1
 Borchert T, 2017, J AM COLL CARDIOL, V70, P975, DOI 10.1016/j.jacc.2017.06.061
 Dybkova N, 2018, CARDIOVASC RES, V114, P1728, DOI 10.1093/cvr/cvy152
 Jabbari J, 2015, CIRC-CARDIOVASC GENE, V8, P64, DOI 10.1161/HCG.0000000000000022
funders: Else-Kroner-Fresenius Stiftung
funders: Deutsche Forschungsgemeinschaft (DFG) through the International Research Training Group Award(German Research Foundation (DFG))
author_address: [Maurer, Wiebke; Hartmann, Nico; Sossalla, Samuel; Streckfuss-Boemeke, Katrin] Georg August Univ Gottingen, Clin Cardiol & Pneumol, Gottingen, Germany; [Maurer, Wiebke; Hartmann, Nico; Argyriou, Loukas; Sossalla, Samuel; Streckfuss-Boemeke, Katrin] DZHK German Ctr Cardiovasc Res, Partner Site Gottingen, Gottingen, Germany; [Argyriou, Loukas] Univ Med Ctr Gottingen UMG, Inst Human Genet, Gottingen, Germany; [Sossalla, Samuel] Univ Med Ctr Regensburg, Dept Internal Med 2, Regensburg, Germany; [Streckfuss-Boemeke, Katrin] Univ Wurzburg, Inst Pharmacol & Toxicol, Versbacher Str 9, D-97078 Wurzburg, Germany
reprint_address: Streckfuss-Bomeke, K (corresponding author), Univ Wurzburg, Inst Pharmacol & Toxicol, Versbacher Str 9, D-97078 Wurzburg, Germany.
funder_text: Else-Kroner-Fresenius Stiftung; Deutsche Forschungsgemeinschaft (DFG) through the International Research Training Group Award [(IRTG) 1816, IRTG 1816]|The authors thank Johanna Heine, Yvonne Metz (Clinic for Cardiology and Pneumology, UMG) and Ilona Eggert (Institute for Human Genetics, UMG) for superb technical support. This work was supported by the Else-Kroner-Fresenius Stiftung (N.H.) and the Deutsche Forschungsgemeinschaft (DFG) through the International Research Training Group Award (IRTG) 1816 (to K.S.B.; W.M. is a fellow under IRTG 1816) .
pubmed_id: 35092938
doi: 10.1016/j.scr.2022.102677
wok_id: 2023-06-27
publication_abbrev: STEM CELL RES
publication_iso: Stem Cell Res.
wos_categories: Cell & Tissue Engineering
wos_categories: Biotechnology & Applied Microbiology
wos_categories: Cell Biology
research_areas: Cell Biology
research_areas: Biotechnology & Applied Microbiology
oa_access: gold
oa_access: Green Published
cor_ur: other
doaj: doaj_with_apc
fp7_project: no
fp7_type: info:eu-repo/semantics/article
citation:   Maurer, Wiebke, Hartmann, Nico, Argyriou, Loukas, Sossalla, Samuel und Streckfuss-Bömeke, Katrin  (2022) Generation of homozygous Nav1.8 knock-out iPSC lines by CRISPR Cas9 genome editing to investigate a potential new antiarrhythmic strategy.  Stem Cell Research 60, S. 102677.