Screening for β-poly(l-malate) binding proteins by affinity chromatography

DOI zum Zitieren dieses Dokuments:: 10.5283/epub.113

Göttler, Thomas ; Holler, Eggehard

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Veröffentlichungsdatum dieses Volltextes: 05 Aug 2009 13:21

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Zusammenfassung

Poly(β-l-malic acid) is a cell type-specific polymer of myxomycetes (true slime molds) with the physiological role to organize mobility of certain proteins over the giant multinucleated plasmodia. We have developed an affinity chromatography employing 1,6-diamino-n-hexane–Sepharose-coupled poly(malic acid) to identify such proteins in cellular extracts of Physarum polycephalum. Molecular masses ...