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Library of prefabricated locked nucleic acid hydrolysis probes facilitates rapid development of reverse-transcription quantitative real-time PCR assays for detection of novel influenza A/H1N1/09 virus.

Wenzel, Jürgen J ; Walch, Heiko ; Bollwein, Markus ; Niller, Hans Helmut ; Ankenbauer, Waltraud ; Mauritz, Ralf ; Höltke, Hans-Joachim ; Zepeda, Héctor Manuel ; Wolf, Hans ; Jilg, Wolfgang ; Reischl, Udo
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Date of publication of this fulltext: 15 Dec 2009 07:34


Abstract

BACKGROUND: The emergence of a novel pandemic human strain of influenza A (H1N1/09) has clearly demonstrated the need for flexible tools enabling the rapid development of new diagnostic methods. METHODS: We designed a set of reverse-transcription quantitative real-time PCR (RT-qPCR) assays based on the Universal ProbeLibrary (UPL)--a collection of 165 presynthesized, fluorescence-labeled locked ...

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