Library of prefabricated locked nucleic acid hydrolysis probes facilitates rapid development of reverse-transcription quantitative real-time PCR assays for detection of novel influenza A/H1N1/09 virus.

Wenzel, Jürgen J, Walch, Heiko, Bollwein, Markus, Niller, Hans Helmut, Ankenbauer, Waltraud, Mauritz, Ralf, Höltke, Hans-Joachim, Zepeda, Héctor Manuel, Wolf, Hans, Jilg, Wolfgang and Reischl, Udo (2009) Library of prefabricated locked nucleic acid hydrolysis probes facilitates rapid development of reverse-transcription quantitative real-time PCR assays for detection of novel influenza A/H1N1/09 virus. Clinical chemistry 55 (12), pp. 2218-22.

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Date of publication of this fulltext: 15 Dec 2009 07:34

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Other URL: http://www.clinchem.org/cgi/content/full/55/12/2218

Abstract

BACKGROUND: The emergence of a novel pandemic human strain of influenza A (H1N1/09) has clearly demonstrated the need for flexible tools enabling the rapid development of new diagnostic methods. METHODS: We designed a set of reverse-transcription quantitative real-time PCR (RT-qPCR) assays based on the Universal ProbeLibrary (UPL)--a collection of 165 presynthesized, fluorescence-labeled locked ...