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Expression, purification and characterization of the enzyme II mannitol-specific domain from Staphylococcus carnosus and determination of the active-site cysteine residue

Pogge von Strandmann, R. ; Weigt, C. ; Fischer, R. ; Meyer, H. E. ; Kalbitzer, Hans Robert ; Hengstenberg, W.


The C-terminal B domain of mannitol-specific enzyme II (enzyme IIB) of the phosphoenolpyruvate-dependent phosphotransferase system for mannitol from Staphylococcus carnosus was subcloned, purified and characterized. In Staphylococcal cells, mannitol-specific enzyme II is composed of a soluble A domain (EIIA) and a transmembrane C domain transporter with a fused enzyme IIB (IIB) domain. We ...


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