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Click Chemistry Based Method for the Preparation of Maleinimide-Type Thiol-Reactive Labels

Link, Martin ; Li, Xiaohua ; Kleim, Jana ; Wolfbeis, Otto S.
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Date of publication of this fulltext: 18 Mar 2011 06:41


Abstract

Maleinimides are widely used to label proteins and to derivatize thiols. The so-called click reaction was shown to allow the efficient introduction of the maleinimido group into azido-modified fluorophores (benzoxazines) by reacting them with an alkyne-modified maleinimide to yield new thiol-reactive fluorescent labels 5–7. The reaction proceeds under mild experimental conditions and provides a ...

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