Negatively cis-acting elements in the distal part of the promoter of Epstein-Barr virus trans-activator gene BZLF1

URN to cite this document:

urn:nbn:de:bvb:355-epub-203915 Copy

Schwarzmann, F. ; Prang, N. ; Reichelt, B. ; Rinkes, B. ; Haist, S. ; Marschall, M. ; Wolf, Hans J.

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Date of publication of this fulltext: 06 Apr 2011 08:04

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Details

Item type:	Article
Journal or Publication Title:	The Journal of general virology
Publisher:	Society for General Microbiology
Volume:	75 ( P
Page Range:	pp. 1999-2006
Date:	1994
Institutions:	Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene
Identification Number:	Value Type 8046403 PubMed ID
Classification:	Notation Type Base Sequence MESH Cells, Cultured MESH DNA Mutational Analysis MESH DNA-Binding Proteins/metabolism MESH Down-Regulation MESH Gene Expression Regulation, Viral MESH Genes, Reporter MESH Herpesvirus 4, Human/growth & development MESH Humans MESH Molecular Sequence Data MESH Promoter Regions, Genetic/genetics MESH Protein Binding MESH Recombinant Fusion Proteins/biosynthesis MESH Trans-Activators/genetics MESH Transfection MESH Viral Proteins MESH Virus Latency/genetics MESH
Dewey Decimal Classification:	600 Technology > 610 Medical sciences Medicine
Status:	Published
Refereed:	Unknown
Created at the University of Regensburg:	Unknown
Item ID:	20391

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Abstract

Epstein-Barr virus (EBV) replicates in a latent or a lytic way in the infected organism, depending on the type and level of differentiation of the host cell. The switch between latency and lytic replication was previously shown, for Burkitt's lymphoma cell lines, to depend on the viral BZLF1 gene product. Protein-DNA assays were used to identify the cis-acting elements that represent the link ...

Abstract

Epstein-Barr virus (EBV) replicates in a latent or a lytic way in the infected organism, depending on the type and level of differentiation of the host cell. The switch between latency and lytic replication was previously shown, for Burkitt's lymphoma cell lines, to depend on the viral BZLF1 gene product. Protein-DNA assays were used to identify the cis-acting elements that represent the link between regulating signal transduction pathways and the viral cascade of gene expression. Specific binding of proteins to several sites of the BZLF1 promoter during latency was shown. Induction of the lytic cycle by stimulation with 12-O-tetradecanoyl-phorbol 13-acetate abolished the binding of these proteins to the distal promoter (positions -227 to -551), suggesting a functional role for the down-regulation of promoter activity during latency. Computer analysis identified a multiply repeated sequence motif, HI, in this region and exonuclease III footprints confirmed that these sites act as specific protein recognition sites. Using a set of reporter plasmids we were able to demonstrate a negative regulatory effect of the HI motif in some B lymphoid cell lines, in contrast to epithelial HeLa cells. The HI silencer elements are different from other silencer elements described so far in respect of their sequence and protein-binding pattern during the activation of BZLF1.

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Metadata last modified: 03 Jun 2018 05:11

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