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In situ calibration of fura-2 and BCECF fluorescence in adult rat ventricular myocytes

Borzak, S., Kelly, R. A., Krämer, Bernhard K., Matoba, Y., Marsh, J. D. and Reers, M. (1990) In situ calibration of fura-2 and BCECF fluorescence in adult rat ventricular myocytes. The American journal of physiology. Heart and circulatory physiology 259 (3 Pt 2), H973-H981.

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Date of publication of this fulltext: 09 Sep 2011 14:41

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Abstract

Quantitation of Ca+ and H+ activities within cells using presently available fluorescent probes is optimal when the fluorescence signal is calibrated in situ after each experiment. Fura-2 and 2',7'-bis(2-carboxy-ethyl)-5,6-carboxyfluoroscein (BCECF) are difficult to calibrate in freshly dissociated adult cardiac myocytes because calibration procedures produce cellular hypercontracture. In situ ...

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Item type:Article
Date:1990
Institutions:UNSPECIFIED
Identification Number:
ValueType
2396701PubMed ID
Classification:
NotationType
Aging/metabolismMESH
AnimalsMESH
Animals, NewbornMESH
Benzofurans/metabolismMESH
Calcium/metabolismMESH
CalibrationMESH
Cells, CulturedMESH
Fluoresceins/metabolismMESH
FluorescenceMESH
Fluorescent DyesMESH
Fura-2MESH
Heart VentriclesMESH
Lanthanum/metabolismMESH
Myocardium/metabolismMESH
Dewey Decimal Classification:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Unknown
Item ID:22058
Owner only: item control page

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