In situ calibration of fura-2 and BCECF fluorescence in adult rat ventricular myocytes

URN to cite this document:: urn:nbn:de:bvb:355-epub-220585
DOI to cite this document:: 10.5283/epub.22058

Borzak, S. ; Kelly, R. A. ; Krämer, Bernhard K. ; Matoba, Y. ; Marsh, J. D. ; Reers, M.

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Date of publication of this fulltext: 09 Sep 2011 14:41

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Abstract

Quantitation of Ca+ and H+ activities within cells using presently available fluorescent probes is optimal when the fluorescence signal is calibrated in situ after each experiment. Fura-2 and 2',7'-bis(2-carboxy-ethyl)-5,6-carboxyfluoroscein (BCECF) are difficult to calibrate in freshly dissociated adult cardiac myocytes because calibration procedures produce cellular hypercontracture. In situ ...